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KMID : 0385520100230020165
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Analytical Science & Technology
2010 Volume.23 No. 2 p.165 ~ p.171
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A glucose biosensor based on deposition of glucose oxidase onto Au nanoparticles poly(maleic anhydride)-grafted multiwalled carbon nanotube electrode
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Piao Ming-Hua
Son Pyeong-Soo
Chang Choo-Hwan
Choi Seong-Ho
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Abstract
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Glucose oxidase (GODox) immobilized biosensor was fabricated by two methods. In one of the methods, gold nanoparticles (Au-NPs) prepared by γ-irradiation were loaded into the poly(maleic anhydride)-grafted multi-walled carbon nanotube, PMAn-g-MWCNT electrode via physical entrapment. In the other method, the Au-NPs were prepared by electrochemical reduction of Au ions on the surface of PMAn-g-MWCNT electrode and then GODox was immobilized into the Au-NPs. The GODox immobilized biosensors were tested for electrocatalytic activities to sense glucose. The sensing range of the biosensor based on the Au-NPs physically modified PMAn-g-MWCNT electrode was from 30 μM to 100 μM for the glucose concentration, and the detection limit was 15 μM. Interferences of ascorbic acid and uric acid were below 7.6%. The physically Au deposited PMAn-g-MWCNT paste electrodes appear to be good sensor in detecting glucose.
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KEYWORD
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maleic anhydride, MWCNT, gold nanoparticles, glucose oxidase, radiation-induced graft polymerization
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